Md (Al-Amin) Chowdhury-Guest Researcher, PhD Student, Department of Pharmacy, University of Helsinki
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Experience
Guest researcher
University of Helsinki
- Pediatric hepatocyte differentiation from iPSCs in normoxia, hypoxia and hyperoxia condition with GDEVS.
- Institution abroad
Mentor
University of Helsinki
- Subject: Immunology
- I did mentor summer intern thesis student during summer time. I had to teach them cell culture, gel run, PCR and immunostaining.
Project: Drug testing on Scleroderma Patient
University of Helsinki
- Scope / workload: 30%
- Funding source: HUS, FIMM of Helsinki
- Scleroderma patients derived fibroblast have been isolated and cultured for the drug testing. More than 500 drugs are now being tested on those cells which takes quite a long time. Several fibroblast markers have been used for characterisation.
Project: APECED PROJECT/ Department of Immunology
University of Helsinki
- Scope / workload: FULL TIME
- Funding source: HUS and Academy of Finland
- Economy / size: Big
- The aim of the project was to develop 'Gene therapy for APECED patients', by delivering correct version of 'AIRE' gene in mutated cells. I started with the APECED disease model rats, genetically modified by Pärt Peterson, University of Tartu. It was a kind gift from Pärt that facilitated us to isolate the Rat epidermal Keratinocyte cells (REK). REK cells were immortalised with commercial SV40 and hTERT Lenti viruses. Immortalised cells have been infected with Endo Aire Lenti virus to correct the mutation with puromycin selection gene. Next plan is to do single cells RNA seq analysis and delivering the corrected cells to Aire -/- rats at Tartu, Estonia.
Research Assistant
HUS & University of Helsinki
- I joined at University of Helsinki as a master's thesis student in 2017 at the department of Obstetrics and Gynaecology, stem cells research project. After completion I continued working as a research assistant at the department of skin and dermatology Department of Skin and Allergy Hospital, HUS. Currently I am also involved in drug research project of FIMM in collaboration with University of Helsinki.
- Institution abroad
Molecular Biology
University of Helsinki
- During my career journey since 2017, I am professional in doing different types of cells culture like H9, keratinocyte, HaCat cells, fibroblasts, human and rat keratinocyte cells, viral transfection and transduction, Axio imaging, EVOS imaging, confocal imaging, molecular analysis such as western blot, immunostaining, DNA, RNA isolation, cDNA synthesis, qPCR, flowcytometry and cells sorting.
- Institution abroad
Project: 'Adipocyte differentiation from MSCs cells'
University of Helsinki
- Scope / workload: Master's thesis student
- Funding source: Academy of Finland
- Economy / size: Big
- My thesis title was 'Adipocyte differentiation from hESCs derived MSCs, comparing two different protocols.' Following two protocols, I successfully differentiated Mesenchymal stem cells from hESCs. Then adipocytes were differentiated from MSCs. Mesenchymal progenitor cells were characterised by flow cytometry analysis, immunostaining and qPCR analysis and adipocytes were evaluated by oil red o staining and qPCR. Later I also explored IL1 alpha, beta, and IL receptor antagonist expression level in different stages of mesenchymal stem cell differentiation. Furthermore, I induced autophagy in MSCs with different concentrations of rapamycin to see if autophagy affects the IL1beta secretion level. Moreover, I also performed a lot of western blot analysis and confocal imaging of different stages of mesenchymal cells. I also collected the supernatant to apply ELISA technique for sensitive analysis of IL1b secretion.
Industry Experience
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Experienced in Biotechnology, Education, Healthcare, and Pharmaceutical.
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Experienced in Research and Development and Product Development.
Skills
- Biopython
- Bioinformatics & Python (Biopython)
- Ms Office
- Excel
- Powerpoint
- Sql
- R
Languages
Education
University of Helsinki
Ph.D. · Clinical Research · Finland
University of Eastern Finland
Biology · Finland · 4.00 out of 4 scale
Mawlana Bhashani Science and Technology University
Biotechnology and Genetic Engineering · Tangail District, Bangladesh · 3.25 out of 4.00
Certifications & licenses
Master's in Biology
University of Eastern Finland
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Experience
Global Experience
Expertise
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Profile
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